Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31195-1-AP targets RAB44 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35126 Product name: Recombinant human RAB44 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of NM_001257357 Sequence: METGQRTSRKVRKLGSNRRRQTREPADGEGAAVAPEPESWSSQAAAELQAFFQDCGAKERGFVTREDLAVAKFSFLGSKEESEMIFDWVDVERKGHLSLEEFSSGLKNIFGSSQSPHRLRRRKPLPSKRVSATTSFPALEEADAEEKEAF 相同性解析による交差性が予測される生物種 |
| Full Name | RAB44, member RAS oncogene family |
| Calculated molecular weight | 111 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | NM_001257357 |
| Gene Symbol | RAB44 |
| Gene ID (NCBI) | 401258 |
| RRID | AB_3669894 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q7Z6P3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab44 is a member of the large Rab GTPase family, encoding an N-terminal EF-hand domain, a coiled-coil domain and a C-terminal Rab GTPase domain. It is highly expressed in immune-related cells such as mast cells, macrophages, osteoclasts and granulocyte lineage cells in the bone marrow. In mouse mast cells, Rab44 is expressed as two isoforms, the long and short forms with molecular masses of 180 kDa and 110 kDa (PMID: 33641252).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB44 antibody 31195-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |