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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RAB43 Monoclonal antibody (66654-1-Ig)

    WB analysis of human brain using 66654-1-Ig (same clone as 66654-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB43 Monoclonal antibody (66654-1-Ig)

    WB analysis using 66654-1-Ig (same clone as 66654-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RAB43 Monoclonal antibody (66654-1-Ig)

    WB analysis of HEK-293 using 66654-1-Ig (same clone as 66654-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66654-1-PBS in a different storage buffer formulation.

RAB43 Monoclonal antibody, PBS Only

RAB43 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66654-1-PBS
Clone No.3B2A8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ISY1, RAB11B, RAB41, Ras related protein Rab 41, Ras related protein Rab 43

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66654-1-PBS targets RAB43 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26911

Product name: Recombinant human RAB43 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 91-212 aa of BC062319

Sequence: ANGAILAYDITKRSSFLSVPHWIEDVRKYAGSNIVQLLIGNKSDLSELREVSLAEAQSLAEHYDILCAIETSAKDSSNVEEAFLRVATELIMRHGGPLFSEKSPDHIQLNSKDIGEGWGCGC

相同性解析による交差性が予測される生物種
Full Name RAB43, member RAS oncogene family
Calculated molecular weight 212 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC062319
Gene Symbol RAB43
Gene ID (NCBI) 339122
RRIDAB_2882011
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ86YS6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ras-related GTP-binding protein 43 (RAB43) is a member of the Ras superfamily. RAB43 is mainly located in endoplasmic reticulum and Golgi, and plays a role as a key regulator of vesicle movement, signal transduction and tethering membrane events in membrane trafficking.

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