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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    WB analysis using 24599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24599-1-AP (RAB3GAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human brain using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human brain using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human cerebellum using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human cerebellum using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

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RAB3GAP2 Polyclonal antibody

RAB3GAP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24599-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24599-1-AP targets RAB3GAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3GAP2 fusion protein Ag20254 相同性解析による交差性が予測される生物種
Full Name RAB3 GTPase activating protein subunit 2 (non-catalytic)
Calculated molecular weight 1393 aa, 156 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC146760
Gene Symbol RAB3GAP2
Gene ID (NCBI) 25782
RRIDAB_2879632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB3GAP2, also known as RAB3-GAP150, belongs to the Rab3-GAP regulatory subunit family. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Rab3 GAP is ubiquitously expressed and enriched in the synaptic soluble fraction of brain, consistent with it having a key role in neurodevelopment. RAB3-GAP150 forms the Rab3 GTPase-activating complex with RAB3-GAP130,where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. Defects in RAB3-GAP150 are the cause of Martsolf and Warburg Micro syndrome.

Protocols

Product Specific Protocols
WB protocol for RAB3GAP2 antibody 24599-1-APDownload protocol
IHC protocol for RAB3GAP2 antibody 24599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols