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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    WB analysis using 66688-1-Ig (same clone as 66688-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    WB analysis of HeLa using 66688-1-Ig (same clone as 66688-1-PBS)

    WB result of RAB3GAP1 antibody (66688-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HeLa cells. This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of mouse brain using 66688-1-Ig (same clone as 66688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of mouse brain using 66688-1-Ig (same clone as 66688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of human testis using 66688-1-Ig (same clone as 66688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of human testis using 66688-1-Ig (same clone as 66688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IF Staining of HEK-293 using 66688-1-Ig (same clone as 66688-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAB3GAP1 antibody (66688-1-Ig, Clone: 4A3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAB3GAP1 Monoclonal antibody, PBS Only

RAB3GAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66688-1-PBS
Clone No.4A3A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

4A3A1, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66688-1-PBS targets RAB3GAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17710

Product name: Recombinant human RAB3GAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 502-851 aa of BC146809

Sequence: PGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWYSPRDYIEEEVIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQIISHSSKVLHFPNPEDKKLEEIIHQITNVEALIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVTGAGRGHAGRIIH

相同性解析による交差性が予測される生物種
Full Name RAB3 GTPase activating protein subunit 1 (catalytic)
Calculated molecular weight 981 aa, 111 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC146809
Gene Symbol RAB3GAP1
Gene ID (NCBI) 22930
RRIDAB_2882042
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15042
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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