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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3C Polyclonal antibody (10788-1-AP)

    WB analysis using 10788-1-AP (same clone as 10788-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using RAB3C Polyclonal antibody (10788-1-AP)

    WB analysis of mouse lung using 10788-1-AP (same clone as 10788-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RAB3C Polyclonal antibody (10788-1-AP)

    WB analysis of human brain using 10788-1-AP (same clone as 10788-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RAB3C Polyclonal antibody (10788-1-AP)

    IP experiment of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    IP result of anti-RAB3C (IP:10788-1-AP, 4ug; Detection:10788-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3C Polyclonal antibody (10788-1-AP)

    IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3C Polyclonal antibody (10788-1-AP)

    IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

RAB3C Polyclonal antibody, PBS Only

RAB3C Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10788-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10788-1-PBS targets RAB3C in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1241

Product name: Recombinant human RAB3C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC013033

Sequence: MRHEAPMQMASAQDARYGQKDSSDQNFDYMFKLLIIGNSSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFKNEKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVILVGNKCDMEDERVISTERGQHLGEQLGFEFFETSAKDNINVKQTFERLVDIICDKMSESLETDPAITAAKQNTRLKETPPPPQPNCAC

相同性解析による交差性が予測される生物種
Full Name RAB3C, member RAS oncogene family
Calculated molecular weight 26 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC013033
Gene Symbol RAB3C
Gene ID (NCBI) 115827
RRIDAB_2177217
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96E17
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.

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