Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 15774-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15774-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15774-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 15774-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15774-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15774-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 15774-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 15774-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of rat brain using 15774-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15774-1-AP (RAB3B/C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 15774-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15774-1-AP (RAB3B/C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15774-1-AP
Immunofluorescent analysis of HeLa cells using 15774-1-AP (RAB3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 15774-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAB3B/C Polyclonal antibody (15774-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

RAB3B/C Polyclonal antibody

RAB3B/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 15774-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

RAB3B, Ras related protein Rab 3B, Ras-related protein Rab-3B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, U-251 cells, rat brain tissue
Positive IHC detected in	rat brain tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15774-1-AP targets RAB3B/C in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8441 Product name: Recombinant human RAB3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC005035 Sequence: MASVTDGKTGVKDASDQNFDYMFKLLIIGNSSVGKTSFLFRYADDTFTPAFVSTVGIDFKVKTVYRHEKRVKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWATQIKTYSWDNAQVILVGNKCDMEEERVVPTEKGQLLAEQLGFDFFEASAKENISVRQAFERLVDAICDKMSDSLDTDPSMLGSSKNTRLSDTPPLLQQNCSC 相同性解析による交差性が予測される生物種
Full Name	RAB3B, member RAS oncogene family
Calculated molecular weight	219 aa, 25 kDa
Observed molecular weight	25 kDa
GenBank accession number	BC005035
Gene Symbol	RAB3B
Gene ID (NCBI)	5865
RRID	AB_2177414
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20337
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
FC protocol for RAB3B/C antibody 15774-1-AP	Download protocol
IF protocol for RAB3B/C antibody 15774-1-AP	Download protocol
IHC protocol for RAB3B/C antibody 15774-1-AP	Download protocol
WB protocol for RAB3B/C antibody 15774-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Ann Neurol PMID: 32167609 Authors - Jimena Monzón-Sandoval View Article
mouse	WB	Hum Mol Genet Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease. Authors - Gaynor A Smith View Article
human	WB,IF	Cell Death Dis Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B Authors - Jinxiu Hu View Article
human	WB	Adv Sci (Weinh) Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase Authors - Johannes Naß View Article
human	IHC	Sci Rep Pan-cancer analysis reveals correlation between RAB3B expression and tumor heterogeneity, immune microenvironment, and prognosis in multiple cancers Authors - Xu-Sheng Liu View Article
human	WB	Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane Authors - Tong Chen KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15774-1-AP

WB analysis using 15774-1-AP

WB analysis using 15774-1-AP

WB analysis of U-251 using 15774-1-AP

WB analysis of mouse brain using 15774-1-AP

WB analysis of human brain using 15774-1-AP

IHC staining of human pancreas using 15774-1-AP

IHC staining of human pancreas using 15774-1-AP

IHC staining of rat brain using 15774-1-AP

IHC staining of rat brain using 15774-1-AP

IF Staining of HeLa using 15774-1-AP

FC experiment of HepG2 using 15774-1-AP

RAB3B/C Polyclonal antibody

RAB3B/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PMID: 32167609

Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.

Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B

Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase

Pan-cancer analysis reveals correlation between RAB3B expression and tumor heterogeneity, immune microenvironment, and prognosis in multiple cancers

Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane