Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of fetal human brain using 12162-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12162-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12162-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12162-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12162-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 12162-1-AP
IP result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:1000) with SH-SY5Y cells lysate 2200 ug.
IP experiment of mouse brain using 12162-1-AP
IP result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:300) with mouse brain tissue lysate 4000ug.
IHC staining of human lymphoma using 12162-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 12162-1-AP (RAB39B antibody) at dilution of 1:50 (under 10x lens).

Featured Product
KD/KO Validated

RAB39B Polyclonal antibody

RAB39B Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12162-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RAB39B, Ras related protein Rab 39B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	fetal human brain tissue, mouse brain tissue, Y79 cells, human brain tissue
Positive IP detected in	SH-SY5Y cells, mouse brain tissue
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 10 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

12162-1-AP targets RAB39B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2803 Product name: Recombinant human RAB39B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-213 aa of BC009714 Sequence: MEAIWLYQFRLIVIGDSTVGKSCLIRRFTEGRFAQVSDPTVGVDFFSRLVEIEPGKRIKLQIWDTAGQERFRSITRAYYRNSVGGLLLFDITNRRSFQNVHEWLEETKVHVQPYQIVFVLVGHKCDLDTQRQVTRHEAEKLAAAYGMKYIETSARDAINVEKAFTDLTRDIYELVKRGEITIQEGWEGVKSGFVPNVVHSSEEVVKSERRCLC 相同性解析による交差性が予測される生物種
Full Name	RAB39B, member RAS oncogene family
Calculated molecular weight	213 aa, 25 kDa
Observed molecular weight	25 kDa
GenBank accession number	BC009714
Gene Symbol	RAB39B
Gene ID (NCBI)	116442
RRID	AB_2177360
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96DA2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for RAB39B antibody 12162-1-AP	Download protocol
IP protocol for RAB39B antibody 12162-1-AP	Download protocol
WB protocol for RAB39B antibody 12162-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy Authors - Shen Zhang KO Validated View Article
mouse	WB	Am J Hum Genet Mutations in RAB39B Cause X-Linked Intellectual Disability and Early-Onset Parkinson Disease with α-Synuclein Pathology. Authors - Gabrielle R Wilson View Article
human	IP	EMBO J Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death. Authors - Chantal Sellier View Article
human	WB	Mov Disord Clinical and Neuropathological Features Associated With Loss of RAB39B. Authors - Yujing Gao View Article
mouse	IF,WB	Acta Neuropathol Commun Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels. Authors - Shangxi Xiao View Article
human	WB,IF	Brain Pathol RAB39B is redistributed in dementia with Lewy bodies and is sequestered within aβ plaques and Lewy bodies. Authors - David J Koss View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of fetal human brain using 12162-1-AP

WB analysis of human brain using 12162-1-AP

WB analysis of mouse brain using 12162-1-AP

WB analysis of Y79 using 12162-1-AP

WB analysis of Y79 using 12162-1-AP

IP experiment of SH-SY5Y using 12162-1-AP

IP experiment of mouse brain using 12162-1-AP

IHC staining of human lymphoma using 12162-1-AP

RAB39B Polyclonal antibody

RAB39B Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Mutations in RAB39B Cause X-Linked Intellectual Disability and Early-Onset Parkinson Disease with α-Synuclein Pathology.

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

Clinical and Neuropathological Features Associated With Loss of RAB39B.

Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels.

RAB39B is redistributed in dementia with Lewy bodies and is sequestered within aβ plaques and Lewy bodies.