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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis of HeLa using 27349-1-AP (same clone as 27349-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis using 27349-1-AP (same clone as 27349-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis of HEK-293 using 27349-1-AP (same clone as 27349-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis of MCF-7 using 27349-1-AP (same clone as 27349-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAB33B Polyclonal antibody (27349-1-AP)

    IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAB33B Polyclonal antibody (27349-1-AP)

    IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

RAB33B Polyclonal antibody, PBS Only

RAB33B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27349-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Ras-related protein Rab-33B, Ras related protein Rab 33B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27349-1-PBS targets RAB33B in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24181

Product name: Recombinant human RAB33B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-229 aa of BC036064

Sequence: IGDSNVGKTCLTYRFCAGRFPDHTEATIGVDFRERAVEIDGERIKIQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNMASFHSLPSWIEECKQHLLANDIPRILVGNKCDLRSAIQVPTDLAQKFADTHSMPLFETSAKNPNDNDHVEAIFMTLAHKLKSHKPLMLSQPPDNGIILKPEPKPAMTCWC

相同性解析による交差性が予測される生物種
Full Name RAB33B, member RAS oncogene family
Calculated molecular weight 229 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC036064
Gene Symbol RAB33B
Gene ID (NCBI) 83452
RRIDAB_2880851
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H082
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAB33B is a small GTP-binding protein of the Rab GTPase family, whose members function in vesicle transport during protein secretion and endocytosis. Rab GTPases are active, membrane-associated proteins that recruit effector proteins in the GTP-bound state and inactive cytosolic proteins when in a GDP-bound state. RAB33B is ubiquitously expressed and has been implicated in Golgi to endoplasmic reticulum cycling of Golgi enzymes. In addition, RAB33B regulates Golgi homeostasis and coordinates intra-Golgi retrograde trafficking.

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