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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB32 Recombinant antibody (82960-1-RR)

    WB analysis using 82960-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82960-1-RR (RAB32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB32 Recombinant antibody (82960-1-RR)

    IF Staining of HeLa using 82960-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB32 antibody (82960-1-RR, Clone: 230315A9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of HeLa cells using RAB32 Recombinant monoclonal antibody (82960-1-RR)

    FC experiment of HeLa using 82960-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAB32 (82960-1-RR, Clone:230315A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of U-251 cells using RAB32 Recombinant monoclonal antibody (82960-1-RR)

    FC experiment of U-251 using 82960-1-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human RAB32 (82960-1-RR, Clone:230315A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 82960-1-RR

    Affinity and Kinetic Characterization of 82960-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82960-1-RR against Human RAB32 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

RAB32 Recombinant monoclonal antibody

RAB32 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82960-1-RR
Clone No.230315A9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

230315A9, Ras related protein Rab 32, Ras-related protein Rab-32

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, C6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-251 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82960-1-RR targets RAB32 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1450

Product name: Recombinant human RAB32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC015061

Sequence: MAGGGAGDPGLGAAAAPAPETREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQCC

相同性解析による交差性が予測される生物種
Full Name RAB32, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC015061
Gene Symbol RAB32
Gene ID (NCBI) 10981
RRIDAB_3670705
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

Protocols

Product Specific Protocols
IF protocol for RAB32 antibody 82960-1-RRDownload protocol
WB protocol for RAB32 antibody 82960-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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