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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB32 Recombinant antibody

RAB32 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230315A9

Cat no : 82960-1-RR

Synonyms

RAB32, Ras related protein Rab 32

Validation Data Gallery

Applications:
  • WB analysis using 82960-1-RR

    WB analysis using 82960-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82960-1-RR (RAB32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 82960-1-RR

    FC experiment of HeLa using 82960-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAB32 (82960-1-RR, Clone:230315A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of U-251 using 82960-1-RR

    FC experiment of U-251 using 82960-1-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human RAB32 (82960-1-RR, Clone:230315A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, C6 cells
Positive FC detected inHeLa cells, U-251 cells
Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

82960-1-RR targets RAB32 in WB, FC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB32 fusion protein Ag1450 相同性解析による交差性が予測される生物種
Full Name RAB32, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC015061
Gene symbol RAB32
Gene ID (NCBI) 10981
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

Protocols

Product Specific Protocols
WB protocol for RAB32 antibody 82960-1-RRDownload protocol
FC protocol for RAB32 antibody 82960-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols