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  • Featured Product
  • KD/KO Validated

RAB32 Polyclonal antibody

RAB32 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10999-1-AP

Synonyms

RAB32, Ras related protein Rab 32

Validation Data Gallery

Applications:
  • WB analysis using 10999-1-AP

    WB analysis using 10999-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 10999-1-AP

    WB analysis using 10999-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human colon using 10999-1-AP

    WB analysis of human colon using 10999-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 10999-1-AP

    IP experiment of HEK-293T using 10999-1-AP

    IP result of anti-RAB32 (IP:10999-1-AP, 4ug; Detection:10999-1-AP 1:500) with HEK-293T cells lysate 1560 ug.

  • IHC staining of human colon using 10999-1-AP

    IHC staining of human colon using 10999-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB32 antibody included
  • IHC staining of human colon using 10999-1-AP

    IHC staining of human colon using 10999-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB32 antibody included
  • IHC staining of human kidney using 10999-1-AP

    IHC staining of human kidney using 10999-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB32 antibody included
  • IHC staining of human kidney using 10999-1-AP

    IHC staining of human kidney using 10999-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB32 antibody included
  • IF Staining of U-251 using 10999-1-AP

    IF Staining of U-251 using 10999-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (10999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, human colon tissue, HepG2 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10999-1-AP targets RAB32 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB32 fusion protein Ag1450 相同性解析による交差性が予測される生物種
Full Name RAB32, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC015061
Gene symbol RAB32
Gene ID (NCBI) 10981
RRIDAB_2177047
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB32 antibody 10999-1-APDownload protocol
IHC protocol for RAB32 antibody 10999-1-APDownload protocol
IF protocol for RAB32 antibody 10999-1-APDownload protocol
IP protocol for RAB32 antibody 10999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cell Death Dis

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission

Authors - Pin Chen
  • KD Validated
View Article