Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83434-2-RR targets RAB31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | RAB31, member RAS oncogene family |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | NM_006868 |
| Gene Symbol | RAB31 |
| Gene ID (NCBI) | 11031 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB31 antibody 83434-2-RR | Download protocol |
| WB protocol for RAB31 antibody 83434-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |