Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 15485-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15485-1-AP
WB result of RAB31 antibody (15485-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB31 transfected HeLa cells.
WB analysis using 15485-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 15485-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 15485-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 15485-1-AP
IP result of anti-RAB31 (IP:15485-1-AP, 4ug; Detection:15485-1-AP 1:1000) with K-562 cells lysate 1640 ug.
IHC staining of human liver cancer using 15485-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15485-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15485-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15485-1-AP (RAB31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RAB31 Polyclonal antibody

RAB31 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15485-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human placenta tissue, HepG2 cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human liver cancer tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

15485-1-AP targets RAB31 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7833 Product name: Recombinant human RAB31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC001148 Sequence: MMAIRELKVCLLGDTGVGKSSIVCRFVQDHFDHNISPTIGASFMTKTVPCGNELHKFLIWDTAGQERFHSLAPMYYRGSAAAVIVYDITKQDSFYTLKKWVKELKEHGPENIVMAIAGNKCDLSDIREVPLKDAKEYAESIGAIVVETSAKNAINIEELFQGISRQIPPLDPHENGNNGTIKVEKPTMQASRRCC 相同性解析による交差性が予測される生物種
Full Name	RAB31, member RAS oncogene family
Calculated molecular weight	22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC001148
Gene Symbol	RAB31
Gene ID (NCBI)	11031
RRID	AB_2176911
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13636
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes.

Protocols

Product Specific Protocols
WB protocol for RAB31 antibody 15485-1-AP	Download protocol
IHC protocol for RAB31 antibody 15485-1-AP	Download protocol
IP protocol for RAB31 antibody 15485-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Transl Med From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses Authors - Tang Biao View Article
human	WB,IF	J Extracell Biol RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer Authors - Yu-Chan Chang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15485-1-AP

WB analysis of HeLa using 15485-1-AP

WB analysis using 15485-1-AP

WB analysis of human placenta using 15485-1-AP