Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 17817-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 17817-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K562 using 17817-1-AP
WB result of RAB27A antibody (17817-1-AP, 1:3000) with si-Control and si-RAB7B transfected K562 cells.
WB analysis of human liver using 17817-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 17817-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17817-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17817-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 17817-1-AP
IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:4000) with HL-60 cells lysate 1200 ug.
IHC staining of human malignant melanoma using 17817-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human malignant melanoma using 17817-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human stomach using 17817-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 17817-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SK-MEL-28 using 17817-1-AP
Immunofluorescent analysis of (4% PFA) fixed SK-MEL-28 cells using RAB27A antibody (17817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

RAB27A Polyclonal antibody

RAB27A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17817-1-AP

Publications(74)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:3.6.5.2, GTP binding protein Ram, GTP-binding protein Ram, Rab 27, RAB27

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (74)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, HL-60 cells, human liver tissue, K562, K-562 cells, L02 cells, THP-1 cells
Positive IP detected in	HL-60 cells
Positive IHC detected in	human malignant melanoma tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SK-MEL-28 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 15 publications below
WB	See 67 publications below
IHC	See 4 publications below
IF	See 10 publications below

Product Information

17817-1-AP targets RAB27A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, pig, canine, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12136 Product name: Recombinant human RAB27A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-221 aa of BC107680 Sequence: MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRASGPDGATGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC 相同性解析による交差性が予測される生物種
Full Name	RAB27A, member RAS oncogene family
Calculated molecular weight	221 aa, 25 kDa
Observed molecular weight	25-27 kDa
GenBank accession number	BC107680
Gene Symbol	RAB27A
Gene ID (NCBI)	5873
RRID	AB_2176728
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51159
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Protocols

Product Specific Protocols
IF protocol for RAB27A antibody 17817-1-AP	Download protocol
IHC protocol for RAB27A antibody 17817-1-AP	Download protocol
IP protocol for RAB27A antibody 17817-1-AP	Download protocol
WB protocol for RAB27A antibody 17817-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Authors - Li Jianhua J View Article
		Blood Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells. Authors - Dawn T Smallwood View Article
human	WB,IF	Nat Neurosci Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models. Authors - Ariel Ionescu View Article
human	WB	Autophagy Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine. Authors - Xiaoyi Chen View Article
human	WB	Autophagy Lipotoxic hepatocyte-derived UBQLN1-enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis. Authors - Fuji Yang View Article
	WB	Nat Commun Pericytes promote metastasis by regulating tumor local vascular tone and hemodynamics Authors - Xiaobo Li View Article

Validation Data Gallery

WB analysis using 17817-1-AP

WB analysis using 17817-1-AP

WB analysis of K562 using 17817-1-AP

WB analysis of human liver using 17817-1-AP

WB analysis of L02 using 17817-1-AP

WB analysis of K-562 using 17817-1-AP

WB analysis of HeLa using 17817-1-AP

IP experiment of HL-60 using 17817-1-AP

IHC staining of human malignant melanoma using 17817-1-AP

IHC staining of human malignant melanoma using 17817-1-AP

IHC staining of human stomach using 17817-1-AP

IHC staining of human stomach using 17817-1-AP

IF Staining of SK-MEL-28 using 17817-1-AP

RAB27A Polyclonal antibody

RAB27A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells.

Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models.

Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine.

Lipotoxic hepatocyte-derived UBQLN1-enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis.

Pericytes promote metastasis by regulating tumor local vascular tone and hemodynamics

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