Validation Data Gallery

Filter: AllWBIHC

WB analysis of HT-29 using 13189-1-AP
HT-29 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 13189-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 13189-1-AP
T-47D cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human renal cell carcinoma using 13189-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 13189-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13189-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13189-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RAB25 Polyclonal antibody

RAB25 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13189-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CATX 8, CATX8, CATX-8, Ras related protein Rab 25, Ras-related protein Rab-25

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-29 cells, Caco-2 cells, T-47D cells
Positive IHC detected in	human renal cell carcinoma tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below
IHC	See 1 publications below

Product Information

13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3864 Product name: Recombinant human RAB25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-213 aa of BC033322 Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACCISL 相同性解析による交差性が予測される生物種
Full Name	RAB25, member RAS oncogene family
Calculated molecular weight	213 aa, 23 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC033322
Gene Symbol	RAB25
Gene ID (NCBI)	57111
RRID	AB_2877921
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P57735
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB25 (RAB25, member RAS oncogene family), also known as RAB11C or CATX-8, is a small GTPase belonging to the RAS superfamily of proteins. RAB25 is a key regulator of receptor recycling and polarized vesicular trafficking in epithelial cells. Unlike many RAB proteins, RAB25 expression is predominantly restricted to epithelial tissues and is frequently dysregulated in carcinomas. It functions as a critical determinant of cellular fate through its context-dependent roles in modulating major signaling pathways, including integrin recycling, growth factor receptor trafficking (e.g., EGFR, IGF1R), and autophagy.

Protocols

Product Specific Protocols
IHC protocol for RAB25 antibody 13189-1-AP	Download protocol
WB protocol for RAB25 antibody 13189-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	FEBS J The protease ADAMTS5 controls ovarian cancer cell invasion, downstream of Rab25 Authors - Shengnan Yuan KD Validated View Article
human	WB	Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane Authors - Tong Chen KD Validated View Article
human	IHC	Transl Cancer Res Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma Authors - Tianyu Zheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HT-29 using 13189-1-AP

WB analysis of Caco-2 using 13189-1-AP

WB analysis of T-47D using 13189-1-AP

IHC staining of human renal cell carcinoma using 13189-1-AP