Various lysates were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

HAP1 (WT and RAB1A and RAB1B KO) lysates prepared with RIPA buffer, 30 μg of protein loaded. 17824-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

WB result of RAB1B antibody (17824-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells. This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

HeLa cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

A375 cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of NIH/3T3 cells using 17824-1-AP (RAB1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB1B antibody (17824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.