Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11671-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
WB analysis using 11671-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.
WB analysis using 11671-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11671-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11671-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 11671-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 11671-1-AP
IP result of anti-RAB1A (IP:11671-1-AP, 4ug; Detection:11671-1-AP 1:4000) with A549 cells lysate 920 ug.
IP experiment of C2C12 using 11671-1-AP
IP result of anti-RAB1A (IP:11671-1-AP, 4ug; Detection:11671-1-AP 1:4000) with C2C12 cells lysate 1280 ug.
IHC staining of human lung cancer using 11671-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11671-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 11671-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11671-1-AP (RAB1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 11671-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11671-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 11671-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11671-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 11671-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB1A antibody (11671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of C2C12 using 11671-1-AP
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using RAB1A antibody (11671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of A549 using 11671-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using RAB1A antibody (11671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of C2C12 using 11671-1-AP
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human RAB1A (11671-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

RAB1A Polyclonal antibody

RAB1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11671-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

YPT1 related protein, YPT1, Ras-related protein Rab-1A, Ras related protein Rab 1A, RAB1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, NIH/3T3 cells, U-251 cells, rat brain tissue, U2OS cells
Positive IP detected in	A549 cells, C2C12 cells
Positive IHC detected in	human lung cancer tissue, human liver tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	C2C12 cells, NIH/3T3 cells, A549 cells
Positive FC (Intra) detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 17 publications below
IHC	See 6 publications below
IF	See 7 publications below
IP	See 4 publications below

Product Information

11671-1-AP targets RAB1A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2264 Product name: Recombinant human RAB1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC000905 Sequence: MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC 相同性解析による交差性が予測される生物種
Full Name	RAB1A, member RAS oncogene family
Calculated molecular weight	205 aa, 23 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC000905
Gene Symbol	RAB1A
Gene ID (NCBI)	5861
RRID	AB_2173437
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62820
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

Protocols

Product Specific Protocols
IF protocol for RAB1A antibody 11671-1-AP	Download protocol
IHC protocol for RAB1A antibody 11671-1-AP	Download protocol
IP protocol for RAB1A antibody 11671-1-AP	Download protocol
WB protocol for RAB1A antibody 11671-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IP	Cancer Cell Rab1A Is an mTORC1 Activator and a Colorectal Oncogene. Authors - Janice D Thomas KD Validated View Article
human	IP	EMBO J Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death. Authors - Chantal Sellier View Article
mouse	WB	Hum Mol Genet Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease. Authors - Gaynor A Smith View Article
human	WB,IHC,IF	Cancer Lett Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer. Authors - Tinglei Huang KD Validated View Article
mouse	WB	Mol Cell Proteomics Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome. Authors - Campbell-Valois François-Xavier FX View Article
mouse	IF	Hum Mol Genet Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms. Authors - Mukesh Gautam View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11671-1-AP

WB analysis using 11671-1-AP

WB analysis using 11671-1-AP

WB analysis of human brain using 11671-1-AP

WB analysis of NIH/3T3 using 11671-1-AP

WB analysis of U-251 using 11671-1-AP

IP experiment of A549 using 11671-1-AP

IP experiment of C2C12 using 11671-1-AP

IHC staining of human lung cancer using 11671-1-AP

IHC staining of human lung cancer using 11671-1-AP

IHC staining of human liver using 11671-1-AP

IHC staining of human liver using 11671-1-AP

IHC staining of human liver using 11671-1-AP

IHC staining of human colon cancer using 11671-1-AP

IHC staining of human colon cancer using 11671-1-AP

IF Staining of NIH/3T3 using 11671-1-AP

IF Staining of C2C12 using 11671-1-AP

IF Staining of A549 using 11671-1-AP

FC experiment of C2C12 using 11671-1-AP

RAB1A Polyclonal antibody

RAB1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.

Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer.

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.