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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB14 Monoclonal antibody (67953-1-Ig)

    WB analysis using 67953-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of pig brain tissue using RAB14 Monoclonal antibody (67953-1-Ig)

    WB analysis of pig brain using 67953-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using RAB14 Monoclonal antibody (67953-1-Ig)

    WB analysis of pig brain using 67953-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HCT 116 cells using RAB14 Monoclonal antibody (67953-1-Ig)

    WB analysis of HCT 116 using 67953-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RAB14 Monoclonal antibody (67953-1-Ig)

    WB analysis of K-562 using 67953-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using RAB14 Monoclonal antibody (67953-1-Ig)

    WB analysis of HSC-T6 using 67953-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAB14 Monoclonal antibody (67953-1-Ig)

    WB analysis of NIH/3T3 using 67953-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using RAB14 Monoclonal antibody (67953-1-Ig)

    IHC staining of mouse ovary using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB14 Monoclonal antibody (67953-1-Ig)

    IHC staining of human colon cancer using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using RAB14 Monoclonal antibody (67953-1-Ig)

    IHC staining of mouse ovary using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using RAB14 Monoclonal antibody (67953-1-Ig)

    IHC staining of mouse ovary using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB14 Monoclonal antibody (67953-1-Ig)

    IF Staining of HeLa using 67953-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB14 antibody (67953-1-Ig, Clone: 3G7B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RAB14 Monoclonal antibody

RAB14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67953-1-Ig
Clone No.3G7B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3G7B3, EC:3.6.5.2, FBP, RAB 14, Ras related protein Rab 14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, pig brain tissue, HCT 116 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse ovary tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67953-1-Ig targets RAB14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB14 fusion protein Ag8167 相同性解析による交差性が予測される生物種
Full Name RAB14, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC006081
Gene Symbol RAB14
Gene ID (NCBI) 51552
RRIDAB_2918705
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB14, the final member of the Rab11 subfamily, participates in the regulation of cell secretion, ensuring the timely release of necessary substances outside the cell. RAB14 is ubiquitously expressed and localizes to the Golgi/TGN and at early endosomes (PMID: 16962593, 22595670). RAB14 contains 215 amino acids, with a molecular weight of approximately 24.6 kDa

Protocols

Product Specific Protocols
WB protocol for RAB14 antibody 67953-1-IgDownload protocol
IHC protocol for RAB14 antibody 67953-1-IgDownload protocol
IF protocol for RAB14 antibody 67953-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols