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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB13 Recombinant antibody (83336-2-RR)

    WB analysis using 83336-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RAB13 Recombinant antibody (83336-2-RR)

    WB analysis of HEK-293 using 83336-2-RR

    WB result of RAB13 antibody (83336-2-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Recombinant antibody (83336-2-RR)

    IF Staining of HeLa using 83336-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Recombinant antibody (83336-2-RR)

    IF Staining of HeLa using 83336-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of K-562 cells using RAB13 Recombinant antibody (83336-2-RR)

    FC experiment of K-562 using 83336-2-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83336-2-RR

    Affinity and Kinetic Characterization of 83336-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83336-2-RR against Human RAB13 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

RAB13 Recombinant monoclonal antibody

RAB13 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83336-2-RR
Clone No.240216D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

GIG4, 240216D10, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, L02 cells, SW480 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83336-2-RR targets RAB13 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34898

Product name: Recombinant human RAB13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-200 aa of BC000799

Sequence: QNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKC

相同性解析による交差性が予測される生物種
Full Name RAB13, member RAS oncogene family
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC000799
Gene Symbol RAB13
Gene ID (NCBI) 5872
RRIDAB_3670998
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP51153
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

Protocols

Product Specific Protocols
IF protocol for RAB13 antibody 83336-2-RRDownload protocol
WB protocol for RAB13 antibody 83336-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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