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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB13 Polyclonal antibody (11718-1-AP)

    WB analysis using 11718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RAB13 Polyclonal antibody (11718-1-AP)

    WB analysis of HEK-293 using 11718-1-AP

    WB result of RAB13 antibody (11718-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using RAB13 Polyclonal antibody (11718-1-AP)

    WB analysis using 11718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAB13 Polyclonal antibody (11718-1-AP)

    IHC staining of mouse testis using 11718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11718-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Polyclonal antibody (11718-1-AP)

    IF Staining of HeLa using 11718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (11718-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of K-562 cells using RAB13 Polyclonal antibody (11718-1-AP)

    FC experiment of K-562 using 11718-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug RAB13 Polyclonal antibody (11718-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

RAB13 Polyclonal antibody

RAB13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11718-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse colon tissue, mouse lung tissue, rat lung tissue, rat colon tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11718-1-AP targets RAB13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2292

Product name: Recombinant human RAB13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-203 aa of BC000799

Sequence: MAKAYDHLFKLLLIGDSGVGKTCLIIRFAEDNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKCSLG

相同性解析による交差性が予測される生物種
Full Name RAB13, member RAS oncogene family
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC000799
Gene Symbol RAB13
Gene ID (NCBI) 5872
RRIDAB_3669132
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51153
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

Protocols

Product Specific Protocols
IF protocol for RAB13 antibody 11718-1-APDownload protocol
IHC protocol for RAB13 antibody 11718-1-APDownload protocol
WB protocol for RAB13 antibody 11718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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