Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 18843-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18843-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 18843-1-AP
IP result of anti-RAB12 (IP:18843-1-AP, 4ug; Detection:18843-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of human kidney using 18843-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18843-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 18843-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 18843-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18843-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18843-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18843-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB12 antibody (18843-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

RAB12 Polyclonal antibody

RAB12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18843-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Ras related protein Rab 12, Ras-related protein Rab-12

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	fetal human brain tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue, mouse brain tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 19 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

18843-1-AP targets RAB12 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	RAB12, member RAS oncogene family
Calculated molecular weight	27 kDa
Observed molecular weight	28 kDa
GenBank accession number	NM_001025300
Gene Symbol	RAB12
Gene ID (NCBI)	201475
RRID	AB_10603469
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6IQ22
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.

Protocols

Product Specific Protocols
IF protocol for RAB12 antibody 18843-1-AP	Download protocol
IHC protocol for RAB12 antibody 18843-1-AP	Download protocol
IP protocol for RAB12 antibody 18843-1-AP	Download protocol
WB protocol for RAB12 antibody 18843-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Curr Biol Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes. Authors - C Alexander Boecker View Article
human	WB	EMBO Rep Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy. Authors - Jie Xu View Article
human	WB	Neurobiol Dis Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment. Authors - Jillian H Kluss View Article
mouse	WB	J Immunol Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex. Authors - Adi Efergan KD Validated View Article
human	WB	Front Oncol Rab12 Promotes Radioresistance of HPV-Positive Cervical Cancer Cells by Increasing G2/M Arrest. Authors - Yujie Huang KD Validated View Article
human	WB	Traffic Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport. Authors - Gustaf E Rydell KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18843-1-AP

WB analysis of HeLa using 18843-1-AP

IP experiment of HeLa using 18843-1-AP

IHC staining of human kidney using 18843-1-AP

IHC staining of human kidney using 18843-1-AP

IHC staining of rat brain using 18843-1-AP

IHC staining of rat brain using 18843-1-AP

IHC staining of mouse brain using 18843-1-AP

IHC staining of mouse brain using 18843-1-AP

IF Staining of HeLa using 18843-1-AP

RAB12 Polyclonal antibody

RAB12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes.

Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy.

Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment.

Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.

Rab12 Promotes Radioresistance of HPV-Positive Cervical Cancer Cells by Increasing G2/M Arrest.

Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.