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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB11A/B Recombinant monoclonal antibody (87237-1-RR)

    WB analysis using 87237-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87237-1-RR (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using RAB11A/B Recombinant monoclonal antibody (87237-1-RR)

    WB analysis of A431 using 87237-1-RR

    WB result of RAB11A/B antibody (87237-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A/B transfected A431 cells.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using RAB11A/B Recombinant monoclonal antibody (87237-1-RR)

    IP experiment of mouse spleen using 87237-1-RR

    IP result of anti-RAB11A/B (IP:87237-1-RR, 4ug; Detection:87237-1-RR 1:3000) with mouse spleen tissue lysate 2000 ug.

  • KD/KO Validated

RAB11A/B Recombinant monoclonal antibody

RAB11A/B Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 87237-1-RR
Clone No.252492E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Rab 11, RAB11, RAB11A, EC:3.6.5.2, Rab-11

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87237-1-RR targets RAB11A/B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8742

Product name: Recombinant human RAB11A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC013348

Sequence: MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI

相同性解析による交差性が予測される生物種
Full Name RAB11A, member RAS oncogene family
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC013348
Gene Symbol RAB11A
Gene ID (NCBI) 8766
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62491
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Protocols

Product Specific Protocols
IP protocol for RAB11A/B antibody 87237-1-RRDownload protocol
WB protocol for RAB11A/B antibody 87237-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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