Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of MDCK using 27094-1-AP
MDCK cells were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 27094-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 27094-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 27094-1-AP
WB result of RAB10 antibody (27094-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB10 transfected HeLa cells.
WB analysis of HeLa using 27094-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 27094-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27094-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 27094-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB10 antibody (27094-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 27094-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RAB10 antibody (27094-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 27094-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RAB10 (27094-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

RAB10 Polyclonal antibody

RAB10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 27094-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

EC:3.6.5.2, Ras related protein Rab 10, Ras-related protein Rab-10

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, HeLa cells, MDCK cells, mouse brain tissue, rat brain tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, MCF-7 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IF	See 1 publications below

Product Information

27094-1-AP targets RAB10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25830 Product name: Recombinant human RAB10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 130-200 aa of BC000896 Sequence: RVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC 相同性解析による交差性が予測される生物種
Full Name	RAB10, member RAS oncogene family
Calculated molecular weight	200 aa, 23 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC000896
Gene Symbol	RAB10
Gene ID (NCBI)	10890
RRID	AB_2880752
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P61026
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB10 is a member of the RAB family of small GTPases, which are key regulators of vesicular trafficking. RAB proteins are cyclically controlled, requiring a GDP-GTP exchange that is facilitated by RAB guanine nucleotide exchange factors. Rab10 plays essential functional roles in development: mice are embryonic lethal when both alleles are deleted. In addition, RAB10 plays a role in maintenance and regulation of endoplasmic reticulum (ER) morphology. RAB10 function has also been linked to neuronal morphology and polarization, playing a critical role in axonal development and dendrite arborization.

Protocols

Product Specific Protocols
FC protocol for RAB10 antibody 27094-1-AP	Download protocol
IF protocol for RAB10 antibody 27094-1-AP	Download protocol
IHC protocol for RAB10 antibody 27094-1-AP	Download protocol
WB protocol for RAB10 antibody 27094-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome. Authors - Tatsuo Miyamoto KO Validated View Article
mouse	IF	Biochem Biophys Res Commun Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation Authors - Miku Fukawa View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MDCK using 27094-1-AP

WB analysis using 27094-1-AP

WB analysis using 27094-1-AP

WB analysis of HeLa using 27094-1-AP

WB analysis of HeLa using 27094-1-AP

IHC staining of human colon using 27094-1-AP

IHC staining of human colon using 27094-1-AP

IF Staining of NIH/3T3 using 27094-1-AP

IF Staining of MCF-7 using 27094-1-AP

FC experiment of HeLa using 27094-1-AP