Validation Data Gallery
Tested Applications
| Positive WB detected in | U-251 cells, human placenta tissue, MCF-7 cells, RT-4 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15926-1-AP targets QRSL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8756 Product name: Recombinant human QRSL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 280-528 aa of BC014389 Sequence: GIPKEYLVPELSSEVQSLWSKAADLFESEGAKVIEVSLPHTSYSIVCYHVLCTSEVASNMARFDGLQYGHRCDIDVSTEAMYAATRREGFNDVVRGRILSGNFFLLKENYENYFVKAQKVRRLIANDFVNAFNSGVDVLLTPTTLSEAVPYLEFIKEDNRTRSAQDDIFTQAVNMAGLPAVSIPVALSNQGLPIGLQFIGRAFCDQQLLTVAKWFEKQVQFPVIQLQELMDDCSAVLENEKLASVSLKQ 相同性解析による交差性が予測される生物種 |
| Full Name | glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 |
| Calculated molecular weight | 528 aa, 57 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC014389 |
| Gene Symbol | QRSL1 |
| Gene ID (NCBI) | 55278 |
| RRID | AB_10638908 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0R6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
QRSL1 is a mitochondrial glutamyl‑tRNA(Gln) amidotransferase subunit A in humans, encoded by nuclear‑located QRSL1 gene. It mediates Gln‑tRNA(Gln) biosynthesis via transamidation, supporting mitochondrial translation and oxidative phosphorylation function. Biallelic pathogenic QRSL1 variants trigger combined oxidative phosphorylation deficiency 40 (COXPD40), a severe autosomal‑recessive mitochondrial disorder with cardiomyopathy, adrenal insufficiency and multi‑organ injury in infants. (PMID: 35894854)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for QRSL1 antibody 15926-1-AP | Download protocol |
| WB protocol for QRSL1 antibody 15926-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |



