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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using QPCT Polyclonal antibody (18571-1-AP)

    WB analysis using 18571-1-AP (same clone as 18571-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18571-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using QPCT Polyclonal antibody (18571-1-AP)

    WB analysis using 18571-1-AP (same clone as 18571-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18571-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using QPCT Polyclonal antibody (18571-1-AP)

    IF Staining of HEK-293 using 18571-1-AP (same clone as 18571-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using QPCT antibody (18571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18571-1-PBS in a different storage buffer formulation.

QPCT Polyclonal antibody, PBS Only

QPCT Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 18571-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC, EC:2.3.2.5, Glutaminyl cyclase, glutaminyl peptide cyclotransferase, Glutaminyl-peptide cyclotransferase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18571-1-PBS targets QPCT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13416

Product name: Recombinant human QPCT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-361 aa of BC047756

Sequence: MAGGRHRRVVGTLHLLLLVAALPWASRGVSPSASAWPEEKNYHQPAILNSSALRQIAEGTSISEMWQNDLQPLLIERYPGSPGSYAARQHIMQRIQRLQADWVLEIDTFLSQTPYGYRSFSNIISTLNPTAKRHLVLACHYDSKYFSHWNNRVFVGATDSAVPCAMMLELARALDKKLLSLKTVSDSKPDLSLQLIFFDGEEAFLHWSPQDSLYGSRHLAAKMASTPHPPGARGTSQLHGMDLLVLLDLIGAPNPTFPNFFPNSARWFERLQAIEHELHELGLLKDHSLEGRYFQNYSYGGVIQDDHIPFLRRGVPVLHLIPSPFPEVWHTMDDNEENLDESTIDNLNKILQVFVLEYLHL

相同性解析による交差性が予測される生物種
Full Name glutaminyl-peptide cyclotransferase
Calculated molecular weight 361 aa, 41 kDa
Observed molecular weight41 kDa, 35 kDa
GenBank accession numberBC047756
Gene Symbol QPCT
Gene ID (NCBI) 25797
RRIDAB_3669301
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16769
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glutaminyl-peptide cyclotransferase (QPCT) gene encodes glutaminyl cyclase (QC), an enzyme that plays a role in post-translational modification of proteins by converting N-terminal glutamate to pyroglutamine. QPCT plays a specific role in promoting tumor cell proliferation, migration and angiogenesis as well as in inhibiting tumor cell apoptosis in cancers such as thyroid cancer and renal cell carcinoma (PMID: 38417050). Human QPCT has two isoforms produced by alternative splicing, 41 kDa and 35 kDa, respectively.

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