Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 12645-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12645-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 12645-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12645-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12645-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 12645-1-AP
IP result of anti-GlnRS (IP:12645-1-AP, 4ug; Detection:12645-1-AP 1:500) with PC-3 cells lysate 3200ug.
IHC staining of human prostate cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat ovary using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-3 using 12645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GlnRS antibody (12645-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HeLa using 12645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GlnRS antibody (12645-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

GlnRS Polyclonal antibody

GlnRS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12645-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

QARS, EC:6.1.1.18, Glutamine--tRNA ligase, Glutaminyl-tRNA synthetase, QARS1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, NIH/3T3 cells, PC-3 cells
Positive IP detected in	PC-3 cells
Positive IHC detected in	human prostate cancer tissue, rat brain tissue, mouse stomach tissue, human placenta tissue, rat ovary tissue, mouse brain tissue, human ovary cancer tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:1500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
RIP	See 1 publications below

Product Information

12645-1-AP targets GlnRS in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3375 Product name: Recombinant human QARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 362-717 aa of BC016634 Sequence: VKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV 相同性解析による交差性が予測される生物種
Full Name	glutaminyl-tRNA synthetase
Calculated molecular weight	775 aa, 88 kDa
Observed molecular weight	88 kDa
GenBank accession number	BC016634
Gene Symbol	GlnRS
Gene ID (NCBI)	5859
RRID	AB_2098676
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P47897
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

Protocols

Product Specific Protocols
IF protocol for GlnRS antibody 12645-1-AP	Download protocol
IHC protocol for GlnRS antibody 12645-1-AP	Download protocol
IP protocol for GlnRS antibody 12645-1-AP	Download protocol
WB protocol for GlnRS antibody 12645-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. Authors - Maria C Passarelli View Article
human	WB	Protein Cell Glutamine signaling specifically activates c-Myc and Mcl-1 to Facilitate cancer cell proliferation and survival Authors - Meng Wang View Article
human	WB	Protein Cell Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation Authors - Kai-Hui Zhang View Article
human	WB	Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation Authors - Raja H Ali View Article
human	WB	Nucleic Acids Res Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex. Authors - Haissi Cui View Article
human	WB,RIP	EMBO Rep The tRNA-like small noncoding RNA mascRNA promotes global protein translation. Authors - Xinping Lu KD Validated View Article

Validation Data Gallery

WB analysis using 12645-1-AP

WB analysis of HepG2 using 12645-1-AP

WB analysis of NIH/3T3 using 12645-1-AP

WB analysis of PC-3 using 12645-1-AP

WB analysis of HeLa using 12645-1-AP

WB analysis of PC-3 using 12645-1-AP

WB analysis of HeLa using 12645-1-AP

IP experiment of PC-3 using 12645-1-AP

IHC staining of human prostate cancer using 12645-1-AP

IHC staining of human prostate cancer using 12645-1-AP

IHC staining of mouse brain using 12645-1-AP

IHC staining of human ovary cancer using 12645-1-AP

IHC staining of human placenta using 12645-1-AP

IHC staining of mouse stomach using 12645-1-AP

IHC staining of rat brain using 12645-1-AP

IHC staining of rat ovary using 12645-1-AP

IHC staining of rat stomach using 12645-1-AP

IF Staining of PC-3 using 12645-1-AP

IF Staining of HeLa using 12645-1-AP

GlnRS Polyclonal antibody

GlnRS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.

Glutamine signaling specifically activates c-Myc and Mcl-1 to Facilitate cancer cell proliferation and survival

Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation

A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation

Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.

The tRNA-like small noncoding RNA mascRNA promotes global protein translation.

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