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Pseudouridine Monoclonal antibody

Pseudouridine Monoclonal Antibody for Dot Blot, ELISA
Cat No. 68578-1-Ig
Clone No.1G7C7

Host / Isotype

Mouse / IgG1

Reactivity

chemical compound

Applications

Dot Blot, ELISA

Ψ, 5-ribosyluracil

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive ELISA detected inPseudouridine
Positive Dot Blot detected inRNA

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:2000-1:20000
DOT BLOTDOT BLOT : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68578-1-Ig targets Pseudouridine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.

Tested Reactivity chemical compound
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTG 相同性解析による交差性が予測される生物種
Full Name Pseudouridine
Gene Symbol
Gene ID (NCBI)
RRIDAB_3085278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pseudouridine is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond. Pseudouridine is the most abundant RNA modification in cellular RNA (PMID: 29104216). After transcription and following synthesis, RNA can be modified with over 100 chemically distinct modifications. These can potentially regulate RNA expression post-transcriptionally, in addition to the four standard nucleotides and play a variety of roles in the cell including translation, localization and stabilization of RNA. Pseudouridine in rRNA and tRNA has been shown to fine-tune and stabilize the regional structure and help maintain their functions in mRNA decoding, ribosome assembly, processing and translation (PMID: 10902565, PMID: 23391857, PMID: 28045575). Pseudouridine in snRNA has been shown to enhance spliceosomal RNA-pre-mRNA interaction to facilitate splicing regulation (PMID 27268497).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68578-1-IgDotBlot.pdf

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