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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Prohibitin Recombinant monoclonal antibody (88442-1-RR)

    WB analysis using 88442-1-RR (same clone as 88442-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88442-1-RR (Prohibitin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88442-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Prohibitin Recombinant monoclonal antibody (88442-1-RR)

    IHC staining of human liver cancer using 88442-1-RR (same clone as 88442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 88442-1-RR (Prohibitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88442-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Prohibitin Recombinant monoclonal antibody (88442-1-RR)

    IHC staining of mouse heart using 88442-1-RR (same clone as 88442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 88442-1-RR (Prohibitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88442-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Prohibitin Recombinant monoclonal antibody (88442-1-RR)

    IHC staining of mouse heart using 88442-1-RR (same clone as 88442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 88442-1-RR (Prohibitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88442-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Prohibitin Recombinant monoclonal antibody (88442-1-RR)

    IF Staining of HeLa using 88442-1-RR (same clone as 88442-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Prohibitin antibody (88442-1-RR, Clone: 260999D9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 88442-1-PBS in a different storage buffer formulation.

Prohibitin Recombinant monoclonal antibody, PBS Only

Prohibitin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 88442-1-PBS
Clone No.260999D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PHB, PHB1, Prohibitin 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88442-1-PBS targets Prohibitin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1240

Product name: Recombinant human Prohibitin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC013401

Sequence: MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ

相同性解析による交差性が予測される生物種
Full Name prohibitin
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC013401
Gene Symbol Prohibitin
Gene ID (NCBI) 5245
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35232
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

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