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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis using 60092-1-Ig (same clone as 60092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60092-1-Ig (Prohibitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis of human placenta using 60092-1-Ig (same clone as 60092-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Prohibitin Monoclonal antibody (60092-1-Ig)

    IF Staining of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunofluorescent analysis of Hela cells, using PHB antibody 60092-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

Prohibitin Monoclonal antibody, PBS Only

Prohibitin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60092-1-PBS
Clone No.4D3G5

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

PHB, 4D3G5, PHB1, Prohibitin 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60092-1-PBS targets Prohibitin in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1240

Product name: Recombinant human Prohibitin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC013401

Sequence: MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ

相同性解析による交差性が予測される生物種
Full Name prohibitin
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC013401
Gene Symbol Prohibitin
Gene ID (NCBI) 5245
RRIDAB_2252403
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35232
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

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