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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using Progesterone Receptor Recombinant antibody (85112-4-RR)

    WB analysis of MCF-7 using 85112-4-RR (same clone as 85112-4-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85112-4-RR (PR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Progesterone Receptor Recombinant antibody (85112-4-RR)

    WB analysis using 85112-4-RR (same clone as 85112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85112-4-RR (Progesterone Receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Progesterone Receptor Recombinant antibody (85112-4-RR)

    IHC staining of human breast cancer using 85112-4-RR (same clone as 85112-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85112-4-RR (Progesterone Receptor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Progesterone Receptor Recombinant antibody (85112-4-RR)

    IHC staining of human cervical cancer using 85112-4-RR (same clone as 85112-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Progesterone Receptor Recombinant antibody (85112-4-RR)

    IHC staining of human cervical cancer using 85112-4-RR (same clone as 85112-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using Progesterone Receptor Recombinant antibody (85112-4-RR)

    IF Staining of T-47D using 85112-4-RR (same clone as 85112-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells, MCF-7 cells, MDA-MB-231 cells using PR antibody (85112-4-RR, Clone: 242686G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85112-4-RR

    Affinity and Kinetic Characterization of 85112-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85112-4-RR against Human Progesterone Receptor were performed. The affinity constant is 0.587 nM.

Progesterone Receptor Recombinant monoclonal antibody, PBS Only

Progesterone Receptor Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85112-4-PBS
Clone No.242686G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PGR, NR3C3, PR, PRGR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85112-4-PBS targets Progesterone Receptor in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag22986

Product name: Recombinant human PR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of NM_000926

Sequence: MTELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAIPISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSSSPPEKDSGLLDSVLDTLLAPSGPGQSQPSPPACEVTSSWCLFGPELPEDPPAAPATQRVLSPLMSRSGCKVGDSSGTAAAHKVLPRGLSPARQLLLPASESPHWSGAPVKPSPQAAAVEVEEEDGSESEESAGPLLKGKPRALGGAAAGGGAAAVPPGAAAGGVALVPKEDSRFSAPRVALVEQDAPMAPGRSPLATTVM

相同性解析による交差性が予測される生物種
Full Name progesterone receptor
Calculated molecular weight 933 aa, 99 kDa
Observed molecular weight 118 kDa
GenBank accession numberNM_000926
Gene Symbol Progesterone Receptor
Gene ID (NCBI) 5241
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06401
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

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