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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (PENK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    Fetal human brain was subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Proenkephalin-A Polyclonal antibody (32272-1-AP)

    IF Staining of SH-SY5Y using 32272-1-AP (same clone as 32272-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Proenkephalin-A antibody (32272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

Proenkephalin-A Polyclonal antibody, PBS Only

Proenkephalin-A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32272-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PENK, Proenkephalin A, Opioid growth factor, Met-enkephalin-Arg-Phe, Met-enkephalin-Arg-Gly-Leu

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32272-1-PBS targets Proenkephalin-A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27972

Product name: Recombinant human PENK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-267 aa of BC032505

Sequence: YGGFMKRYGGFMKKMDELYPMEPEEEANGSEILAKRYGGFMKKDAEEDDSLANSSDLLKELLETGDNRERSHHQDGSDNEEEVSKRYGGFMRGLKRSPQLEDEAKELQKRYGGFMRRVGRPEWWMDYQKRYGGFLKRFAEALPSDEEGESYSKEVPEMEKRYGGFMRF

相同性解析による交差性が予測される生物種
Full Name proenkephalin
Calculated molecular weight 267 aa, 31 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC032505
Gene Symbol Proenkephalin A
Gene ID (NCBI) 5179
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP01210
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Proenkephalin-A (PENK) is also named as Synenkephalin, Met-enkephalin and Opioid growth factor (OGF), and belongs to the opioid neuropeptide precursor family. PENK is a neuropeptide that competes with and mimics the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (PMID:7057924). PENK hypermethylation is also associated with bladder cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer (PMID:36403035). Proenkephalin (PENK) represents a new candidate to determine kidney function. This peptide is cleaved from the precursor peptide preproenkephalin A alongside enkephalins (endogenous opioids) and is filtrated in the glomerulus (PMID: 33636859).

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