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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of TT cells using CALCA/Calcitonin Recombinant antibody (83774-1-RR)

    IF Staining of TT using 83774-1-RR (same clone as 83774-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed TT cells using Procalcitonin/CALCA antibody (83774-1-RR, Clone: 240753G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).(negative control: HeLa cells) This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of TT cells using CALCA/Calcitonin Recombinant antibody (83774-1-RR)

    FC experiment of TT using 83774-1-RR (same clone as 83774-1-PBS)

    1x10^6 TT cells were intracellularly stained with 0.25 ug Procalcitonin/CALCA Recombinant Antibody (83774-1-RR, Clone:240753G2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83774-1-PBS

    Affinity and Kinetic Characterization of 83774-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83774-1-PBS against Human CALCA/Calcitonin was performed. The affinity constant is 47.7 nM.

  • Affinity and Kinetic Characterization of 83774-1-RR

    Affinity and Kinetic Characterization of 83774-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83774-1-RR against Human CALCA/CGRP were performed. The affinity constant is 0.189 nM.

CALCA/Calcitonin Recombinant monoclonal antibody, PBS Only

CALCA/Calcitonin Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83774-1-PBS
Clone No.240753G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Procalcitonin/CALCA, CALCA, Calcitonin, 240753G2, CALC1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83774-1-PBS targets CALCA/Calcitonin in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1060

Product name: Recombinant Human Procalcitonin/CALCA protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-141 aa of NM_001741.3

Sequence: APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN

相同性解析による交差性が予測される生物種
Full Name Calcitonin
Calculated molecular weight15kDa
GenBank accession numberNM_001741.3
Gene Symbol CALCA
Gene ID (NCBI) 796
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01258
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.