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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using Polyglutamine Monoclonal antibody (65239-1-Ig)

    WB analysis of Recombinant protein using 65239-1-Ig (same clone as 65239-1-PBS)

    Western blot analysis of anti-polyglutamine antibody (MW1) binding to huntingtin exon 1 fusion proteins with variable numbers of glutamines. MW1 bound to huntingtin exon 1 proteins with normal and expanded polyQ repeats but did not bind the TRX tag control. (Owens, Gwen E et al. J Mol Biol. 2015 Jul 31;427(15):2507-2519.) This data was developed using the same antibody clone with 65239-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using Polyglutamine Monoclonal antibody (65239-1-Ig)

    ELISA experiment of Recombinant protein using 65239-1-Ig (same clone as 65239-1-PBS)

    Indirect ELISA was carried out by coating polyglutamine containing ASCL1 fusion protein (Cat.NO. Ag26337, containing polyglutamine region) and GST fusion protein expressed from the same vector (Cat.NO. Ag25094, for control) at 70 ng/well followed by blocking and adding serial diluted Polyglutamine mouse monoclonal antibody 65239-1-Ig. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 65239-1-PBS in a different storage buffer formulation.

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Polyglutamine Monoclonal antibody, PBS Only

Polyglutamine Monoclonal Antibody for WB, ELISA, Indirect ELISA
Cat No. 65239-1-PBS
Clone No.MW1

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

n/a

Applications

WB, ELISA, Indirect ELISA

Synonyms

Glutamine repeats, polyQ

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65239-1-PBS targets Polyglutamine in WB, ELISA, Indirect ELISA applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Polyglutamine
GenBank accession numberN/A
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Huntington's disease is a neurodegenerative disorder caused by the expansion of a polyglutamine (polyQ) repeat in the N-terminal portion of huntingtin protein to a length above 35-40 units (PMID: 26047735; 19507258). The mutational expansion of polyglutamine above a critical length causes a toxic gain of function in huntingtin and results in neuronal death. In the course of the disease, expanded huntingtin is proteolyzed, becomes abnormally folded, and accumulates in oligomers, fibrils, and microscopic inclusions (PMID: 25336039). The anti-polyglutamine (polyQ) antibody MW1 specifically binds the polyQ domain of huntingtin exon 1. On western blot, the MW1 clone strongly prefers to bind to the expanded polyQ repeat form of Htt, displaying no detectable binding to normal huntingtin (PMID: 11719267).