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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Polycystin 2 Polyclonal antibody (19126-1-AP)

    WB analysis using 19126-1-AP (same clone as 19126-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Polycystin 2 Polyclonal antibody (19126-1-AP)

    WB analysis of HEK-293 using 19126-1-AP (same clone as 19126-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Polycystin 2 Polyclonal antibody (19126-1-AP)

    WB analysis of human kidney using 19126-1-AP (same clone as 19126-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using Polycystin 2 Polyclonal antibody (19126-1-AP)

    IP experiment of mouse testis using 19126-1-AP (same clone as 19126-1-PBS)

    IP result of anti-Polycystin 2 (IP:19126-1-AP, 4ug; Detection:19126-1-AP 1:500) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Polycystin 2 Polyclonal antibody (19126-1-AP)

    IHC staining of human kidney using 19126-1-AP (same clone as 19126-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Polycystin 2 Polyclonal antibody (19126-1-AP)

    IHC staining of human kidney using 19126-1-AP (same clone as 19126-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Polycystin 2 Polyclonal antibody (19126-1-AP)

    IF Staining of MDCK using 19126-1-AP (same clone as 19126-1-PBS)

    Immunofluorescent analysis of MDCK cells using 19126-1-AP (Polycystin 2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

Polycystin 2 Polyclonal antibody, PBS Only

Polycystin 2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 19126-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19126-1-PBS targets Polycystin 2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name polycystic kidney disease 2 (autosomal dominant)
Calculated molecular weight 110 kDa
Observed molecular weight 109 kDa
GenBank accession numberNM_000297
Gene Symbol PKD2
Gene ID (NCBI) 5311
RRIDAB_10641050
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13563
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221)

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