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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of A549 using 10602-1-AP (same clone as 10602-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of human brain using 10602-1-AP (same clone as 10602-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of HEK-293 using 10602-1-AP (same clone as 10602-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of HeLa using 10602-1-AP (same clone as 10602-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of mouse ovary using 10602-1-AP (same clone as 10602-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of human brain using 10602-1-AP (same clone as 10602-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Plexin-B2 Polyclonal antibody (10602-1-AP)

    WB analysis of MCF-7 using 10602-1-AP (same clone as 10602-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IP experiment of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

    IP result of anti-Plexin B2 (IP:10602-1-AP, 4ug; Detection:10602-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human liver cancer using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human liver cancer using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human breast cancer using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human breast cancer using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human gliomas using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human gliomas using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Plexin-B2 Polyclonal antibody (10602-1-AP)

    IF Staining of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 10602-1-AP (Plexin B2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Plexin-B2 Polyclonal antibody, PBS Only

Plexin-B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10602-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Plexin B2, PLXNB2, KIAA0315, MM1, Nbla00445

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10602-1-PBS targets Plexin-B2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0941

Product name: Recombinant human PLXNB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1542-1841 aa of BC004542

Sequence: WKRVNTLMHYNVRDGATLILSKVGVSQQPEDSQQDLPGERHALLEEENRVWHLVRPTDEVDEGKSKRGSVKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLAPGHAVPPAVKYFFDFLDEQAEKHNIQDEDTIHIWKTNSLPLRFWVNILKNPHFIFDVHVHEVVDASLSVIAQTFMDACTRTEHKLSRDSPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTDSLNTLVALHQLYQYTQKYYDEIINALEEDPAAQKMQLAFRLQQIAAALENKVTDL

相同性解析による交差性が予測される生物種
Full Name plexin B2
Calculated molecular weight 205 kDa
Observed molecular weight 80 kDa, 240 kDa
GenBank accession numberBC004542
Gene Symbol PLXNB2
Gene ID (NCBI) 23654
RRIDAB_2166424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15031
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.

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