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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Plakophilin 1 Recombinant antibody (85217-4-RR)

    WB analysis using 85217-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using Plakophilin 1 Recombinant antibody (85217-4-RR)

    WB analysis of mouse skin using 85217-4-RR

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85217-4-RR

    Affinity and Kinetic Characterization of 85217-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85217-4-RR against Human Plakophilin 1 were performed. The affinity constant is 11.4 nM.

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Plakophilin 1 Recombinant antibody

Plakophilin 1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85217-4-RR
Clone No.242834G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PKP1, 242834G8, B6P, Plakophilin-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skin tissue, HEK-293 cells, A431 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85217-4-RR targets Plakophilin 1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Plakophilin 1 fusion protein Ag18153 相同性解析による交差性が予測される生物種
Full Name plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
Calculated molecular weight 747 aa, 83 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC114571
Gene Symbol PKP1
Gene ID (NCBI) 5317
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13835
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).

Protocols

Product Specific Protocols
WB protocol for Plakophilin 1 antibody 85217-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols