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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Pinin Polyclonal antibody (18266-1-AP)

    WB analysis using 18266-1-AP (same clone as 18266-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Pinin Polyclonal antibody (18266-1-AP)

    IP experiment of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    IP result of anti-Pinin (IP:18266-1-AP, 4ug; Detection:18266-1-AP 1:300) with HeLa cells lysate 520ug. This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pinin Polyclonal antibody (18266-1-AP)

    IF Staining of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Pinin antibody (18266-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pinin Polyclonal antibody (18266-1-AP)

    IF Staining of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Pinin antibody (18266-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Pinin Polyclonal antibody, PBS Only

Pinin Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 18266-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

SR-like protein, PNN, DRS, Domain-rich serine protein, Desmosome-associated protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18266-1-PBS targets Pinin in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pinin fusion protein Ag6643 相同性解析による交差性が予測される生物種
Full Name pinin, desmosome associated protein
Calculated molecular weight 82 kDa
Observed molecular weight 82 kDa, 140 kDa
GenBank accession numberBC062602
Gene Symbol Pinin
Gene ID (NCBI) 5411
RRIDAB_10642138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H307
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PNN, also named as DRS or 140 kDa nuclear and cell adhesion-related phosphoprotein, is a 717 amino acid protein, which belongs to the pinin family. PNN localizes in the plasma membrane and is expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. PNN is expressed strongly in melanoma metastasis lesions and advanced primary tumors. PNN as a transcriptional activator binds to the E-box 1 core sequence of the E-cadherin promoter gene. PNN is involved in the establishment and maintenance of epithelia cell-cell adhesion. PNN is a potential tumor suppressor for renal cell carcinoma. The calculated molecular weight of PNN is 82 kDa (native form), but the post-modified protein is 140 kDa (phospho form of isoform 1).