Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87103-1-RR targets Phospho-TAU (Thr212/Ser214) in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule-associated protein tau |
| Calculated molecular weight | 37-46, 79-81 kDa |
| Observed molecular weight | 45-60 kDa |
| GenBank accession number | BC000558 |
| Gene Symbol | TAU |
| Gene ID (NCBI) | 4137 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The double phosphorylation of Tau at Thr212/Ser214 is a hallmark of Alzheimer's disease neurofibrillary tangles. This modification is critical for inducing a pathological conformation that promotes Tau aggregation into toxic filaments while impairing its normal microtubule-stabilizing function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TAU (Thr212/Ser214) antibody 87103-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |