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Tested Applications
| Positive WB detected in | pervanadate treated Ramos cells |
| Positive IF/ICC detected in | pervanadate treated Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83331-1-RR targets Phospho-SYK (Tyr323) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | spleen tyrosine kinase |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC001645 |
| Gene Symbol | SYK |
| Gene ID (NCBI) | 6850 |
| ENSEMBL Gene ID | ENSG00000165025 |
| RRID | AB_3670995 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P43405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-SYK (Tyr323) antibody 83331-1-RR | Download protocol |
| WB protocol for Phospho-SYK (Tyr323) antibody 83331-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
