Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
87893-2-PBS targets Phospho-STK11/LKB1 (Ser334) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | serine/threonine kinase 11 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC007981 |
| Gene Symbol | LKB1 |
| Gene ID (NCBI) | 6794 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15831 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. Phospho-STK11 (Ser334), also called p-LKB1 (S334), is an inhibitory phosphorylation modification of the tumor suppressor kinase LKB1. This site is phosphorylated by Akt and PIM kinases upon growth factor stimulation. Phosphorylated Ser334 recruits 14-3-3 proteins, sequestering LKB1 in the nucleus and disrupting its binding to STRADα-MO25 complex. This abolishes LKB1's capacity to activate AMPK, impairing energy sensing, autophagy and anti-proliferative signals. Elevated p-LKB1 (Ser334) accelerates proliferation in breast, prostate and lung cancers, weakens metabolic stress response, and correlates with poor tumor prognosis. It serves as a biomarker for aberrant PI3K/Akt oncogenic signaling and represents a target to restore LKB1 tumor-suppressive function. (PMID: 22611470, 34193159)