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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT6 (Tyr641) Recombinant monoclonal anti (86641-1-RR)

    WB analysis using 86641-1-RR

    Non-treated ACHN cells and IL-4 (HZ-1004) treated ACHN cells were subjected to SDS PAGE followed by western blot with 86641-1-RR (Phospho-STAT6 (Tyr641) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of ACHN cells using Phospho-STAT6 (Tyr641) Recombinant monoclonal anti (86641-1-RR)

    IF Staining of ACHN using 86641-1-RR

    Immunofluorescent analysis of (4% PFA) fixed IL-4 treated ACHN cells using Phospho-STAT6 (Tyr641) antibody (86641-1-RR, Clone: 250940C12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of ACHN cells using Phospho-STAT6 (Tyr641) Recombinant monoclonal anti (86641-1-RR)

    FC experiment of ACHN using 86641-1-RR

    1X10^6 ACHN cells untreated (dashed lines) or treated with IL-4 treated which intracellularly stained with 0.13 ug Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody (86641-1-RR, Clone:250940C12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody

Phospho-STAT6 (Tyr641) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 86641-1-RR
Clone No.250940C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

p STAT6 (Tyr641), Phospho STAT6, Phospho STAT6 (Tyr641), Phospho STAT6(Tyr641), Phospho-STAT6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inIL-4 treated ACHN cells
Positive IF/ICC detected inIL-4 treated ACHN cells
Positive FC (Intra) detected inIL-4 treated ACHN cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86641-1-RR targets Phospho-STAT6 (Tyr641) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight 100 kDa
Observed molecular weight110 kDa
GenBank accession numberBC005823
Gene Symbol STAT6
Gene ID (NCBI) 6778
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP42226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. p-STAT6(Tyr641) is a biomarker of Th2-high inflammation, targeted by IL-4Rα blockers (dupilumab) and JAK1/3 inhibitors. High p-STAT6 in TAMs correlates with M2 polarization and radioresistance, blocking IL-4 or JAK1/3 decreases Tyr641 phosphorylation and restores anti-tumor immunity. (PMID: 37474750)

Protocols

Product Specific Protocols
FC protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RRDownload protocol
IF protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RRDownload protocol
WB protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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