Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, MCF-7 cells, EGF treated A549 cells, UV treated HeLa cells, TNF alpha treated MCF-7 cells |
| Positive IF/ICC detected in | UV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa cells |
| Positive FC (Intra) detected in | UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 21 publications below |
| IF | See 3 publications below |
Product Information
60479-1-Ig targets Phospho-STAT3 (Ser727) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
| Calculated molecular weight | 770 aa, 88 kDa |
| Observed molecular weight | 85-90 kDa |
| GenBank accession number | BC000627 |
| Gene Symbol | STAT3 |
| Gene ID (NCBI) | 6774 |
| RRID | AB_3670262 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-STAT3 (Ser727) antibody 60479-1-Ig | Download protocol |
| WB protocol for Phospho-STAT3 (Ser727) antibody 60479-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Mol Biosci Glycoprotein M6B suppresses the maintenance of glioma stem cell stemness and proliferation via the integrin β1/β-catenin pathway. | ||
Cell Signal Oncogenic role of PLOD3 mediated by SOX9 through IL-6/JAK/STAT3 pathway in cervical cancer. | ||
Pharmaceutics MOF-Engineered Platelet-Mimicking Nanocarrier-Encapsulated Cascade Enzymes for ROS Scavenging and Anti-Inflammation in Cerebral Ischemia-Reperfusion Injury. | ||
Pharmaceutics Dual-Action Tocilizumab-Conjugated Cisplatin Nanoparticles Overcome Chemoresistance and Metastasis in Non-Small-Cell Lung Cancer | ||
J Agric Food Chem Ginsenoside Rk3 Alleviates Neuroinflammation and Gastrointestinal Dysfunction in Parkinson's Disease via Modulation of Gut Microbiota-Mediated Butyric Acid Metabolism. | ||
J Inflamm Res Potential Targets and Mechanisms of Saikosaponin D in Psoriasis: A Bioinformatic and Experimental Study on Oxidative Stress. |