Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
84036-4-PBS targets Phospho-RIPK1 (Ser166) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Calculated molecular weight | 76 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | NM_003804 |
| Gene Symbol | RIPK1 |
| Gene ID (NCBI) | 8737 |
| RRID | AB_3743515 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13546 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
RIPK1, a 74 kDa protein, is composed of a N-terminal kinase domain, an intermediate domain (containing the RIP homotypic interaction motif, RHIM) and a C-terminal death domain. Stimulation of cells with TNFα can promote distinct cell death pathways, including RIPK1-independent apoptosis, necroptosis, and RIPK1-dependent apoptosis (RDA). TNFα induces cell necroptosis and the phosphorylation of RIPK1 at the Ser166 residue i.e. p-RIPK1 (Ser166), both of which can be effectively inhibited by Nec-1. Therefore, p-RIPK1 (Ser166) is considered a biomarker for the activation of RIPK1 kinase and necroptosis (PMID: 31440386, PMID: 29891719).