• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-RB1 (Ser807/811) Polyclonal antibody

Phospho-RB1 (Ser807/811) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30376-1-AP

Synonyms

OSRC, p105 Rb, Phospho-RB1 (Ser807/811), pp110, pRb, RB, RB1, retinoblastoma 1

Validation Data Gallery

Applications:
  • WB analysis using 30376-1-AP

    WB analysis using 30376-1-AP

    Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 30376-1-AP (Phospho-RB1 (Ser807/811) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RB1 antibody (10048-2-Ig) subsequently.

Tested Applications

Positive WB detected inCalyculin A treated Jurkat cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

30376-1-AP targets Phospho-RB1 (Ser807/811) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name retinoblastoma 1
Calculated molecular weight 928 aa, 106 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC039060
Gene symbol RB1
Gene ID (NCBI) 5925
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. Phosphorylation and dephosphorylation of RB1 is well known to be a key regulator in cell cycle progression in cancer cells. Phosphorylation of RB1 at multiple S/T residues, first by cyclin D-dependent kinases and subsequently by cyclin E-CDK2 in later G1 phase, completely inactivates RB1, enabling the release of E2F from RB1 complexes and activation of E2F-mediated transcription. Monomethylation at Lys810 by SMYD2 enhances phosphorylation at Ser807 and Ser811, and promotes cell cycle progression. (PMID: 22787429, PMID: 21115810, PMID: 32344731)

Protocols

Product Specific Protocols
WB protocol for Phospho-RB1 (Ser807/811) antibody 30376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols