Validation Data Gallery
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Tested Applications
| Positive WB detected in | Calyculin A treated HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87211-3-RR targets Phospho-RB1 (Ser608) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | retinoblastoma 1 |
| Calculated molecular weight | 928 aa, 106 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC039060 |
| Gene Symbol | RB1 |
| Gene ID (NCBI) | 5925 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P06400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. Ser608 is located within the "spacer" region of RB1, which connects the A and B pocket domains. This region is critical for RB1's interaction with various cellular proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RB1 (Ser608) antibody 87211-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |