Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88430-1-RR targets Phospho-RAF1 (Ser259) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-raf-1 murine leukemia viral oncogene homolog 1 |
| Calculated molecular weight | 648 aa, 73 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC018119 |
| Gene Symbol | RAF1 |
| Gene ID (NCBI) | 5894 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04049 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Phospho-RAF1 (Ser259) is a key inhibitory modification of CRAF within the RAS-MEK-ERK cascade. Phosphorylated mainly by PKA and Akt, this residue recruits 14-3-3 proteins to lock RAF1 in an autoinhibited cytoplasmic conformation, blocking its membrane recruitment and binding to GTP-Ras. Dephosphorylation by PP2A relieves suppression to trigger ERK-driven proliferation. Elevated p-RAF1 (S259) dampens oncogenic MAPK signaling in tumors, while loss of this phosphorylation causes sustained pathway hyperactivation linked to Noonan syndrome and multiple malignancies. (PMID: 11971957, 11756411)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RAF1 (Ser259) antibody 88430-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |