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Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
29107-1-PBS targets Phospho-PKN1 (Thr774) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase N1 |
| Calculated molecular weight | 942 aa, 104 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC040061 |
| Gene Symbol | PKN1 |
| Gene ID (NCBI) | 5585 |
| RRID | AB_2918235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16512 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)