Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
| Positive FC (Intra) detected in | λ phosphatase treated A549 cells |
This antibody is equivalent to Ser643 in mice.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87213-1-RR targets Phospho-PKC Delta (Ser645) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase C, delta |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | NM_006254 |
| Gene Symbol | PKC Delta |
| Gene ID (NCBI) | 5580 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q05655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ/ι), which respond to neither DAG nor calcium. Phospho-PKC Delta (Ser645) refers to the phosphorylated form of Protein Kinase C Delta (PKCδ) at serine residue 645. This phosphorylation site is located in the C-terminal tail of PKCδ, specifically in the turn motif region, and serves as a critical "priming" phosphorylation that regulates enzyme stability and catalytic activity. (PMID: 28784722) This antibody is equivalent to Ser643 in mice.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-PKC Delta (Ser645) antibody 87213-1-RR | Download protocol |
| WB protocol for Phospho-PKC Delta (Ser645) antibody 87213-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |