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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PKC Alpha (Ser657) Recombinant antibody (83840-7-RR)

    WB analysis using 83840-7-RR

    Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 83840-7-RR (Phospho-PKC Alpha (Ser657) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-PKC Alpha (Ser657) Recombinant antibody (83840-7-RR)

    WB analysis using 83840-7-RR

    Non-treated NIH/3T3 cells and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83840-7-RR (Phospho-PKC Alpha (Ser657) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-PKC Alpha (Ser657) Recombinant antibody (83840-7-RR)

    WB analysis using 83840-7-RR

    Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83840-7-RR (Phospho-PKC Alpha (Ser657) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

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Phospho-PKC Alpha (Ser657) Recombinant antibody

Phospho-PKC Alpha (Ser657) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83840-7-RR
Clone No.250276F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

p PKC α (Ser 657), Phospho PKC Alpha, Phospho-PKC Alpha, Phospho-PKC Alpha(Ser657), Phospho-PKC Alpha-Ser657

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCalyculin A treated HSC-T6 cells, PMA treated NIH/3T3 cells, Calyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83840-7-RR targets Phospho-PKC Alpha (Ser657) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase C, alpha
Calculated molecular weight 77 kDa
Observed molecular weight77 kDa
GenBank accession numberAK055431
Gene Symbol PKC Alpha
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17252
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

Protocols

Product Specific Protocols
WB protocol for Phospho-PKC Alpha (Ser657) antibody 83840-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols