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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser15) Polyclonal antibody (28961-1-AP)

    WB analysis using 28961-1-AP (same clone as 28961-1-PBS)

    Non-treated HT-29 and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53(Ser15) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser15) Polyclonal antibody (28961-1-AP)

    WB analysis using 28961-1-AP (same clone as 28961-1-PBS)

    Non-treated HSC-T6 cells and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Phospho-P53 (Ser15) Polyclonal antibody (28961-1-AP)

    IF Staining of HT-29 using 28961-1-AP (same clone as 28961-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed non-treated HT-29 and etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Phospho-P53 (Ser15) Polyclonal antibody (28961-1-AP)

    IF Staining of HT-29 using 28961-1-AP (same clone as 28961-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Phospho-P53 (Ser15) Polyclonal antibody (28961-1-AP)

    IF Staining of HT-29 using 28961-1-AP (same clone as 28961-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

Phospho-P53 (Ser15) Polyclonal antibody, PBS Only

Phospho-P53 (Ser15) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28961-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

p P53, p P53 (Ser15), p P53 Ser15, P53 (Ser15), Phospho P53

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28961-1-PBS targets Phospho-P53 (Ser15) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight53 kDa
GenBank accession numberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
RRIDAB_2881236
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04637
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.

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