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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-NPM1 (Thr199) Recombinant monoclonal antib (87394-1-RR)

    WB analysis of HeLa using 87394-1-RR

    Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87394-1-RR (Phospho-NPM1 (Thr199) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • SEC-HPLC of 87394-1-RR

    SEC-HPLC of 87394-1-RR

    The purity of Human Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (Cat. No. 87394-1-RR) was greater than 95% as determined by SEC-HPLC.

  • Flow cytometry (FC) experiment of Jurkat cells using Phospho-NPM1 (Thr199) Recombinant monoclonal antib (87394-1-RR)

    FC experiment of Jurkat using 87394-1-RR

    1x10^6 Jurkat cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (87394-1-RR, Clone:252639G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-NPM1 (Thr199) Recombinant monoclonal antibody

Phospho-NPM1 (Thr199) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 87394-1-RR
Clone No.252639G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

NPM1 (Thr199), Phospho NPM1 (Thr199), Phospho-NPM, Phospho-NPM (Thr199), Phospho-NPM1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpaclitaxel treated HeLa cells
Positive FC (Intra) detected inpaclitaxel treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87394-1-RR targets Phospho-NPM1 (Thr199) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated molecular weight 33 kDa
Observed molecular weight40 kDa
GenBank accession numberNM_002520
Gene Symbol NPM1
Gene ID (NCBI) 4869
RRIDAB_3745512
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). Phospho-NPM1 (Thr199) is a phosphorylated form of nucleophosmin (NPM1) at threonine 199, a residue that plays a critical role in cell cycle regulation and cancer development. Indeed, phosphorylation by CDK2/cyclin E on Thr199 residue during G1 phase is critical for the NPM1 dissociation from the centrosome to initiate its duplication and the pre-mRNA processing. (PMID: 26993766)

Protocols

Product Specific Protocols
FC protocol for Phospho-NPM1 (Thr199) antibody 87394-1-RRDownload protocol
WB protocol for Phospho-NPM1 (Thr199) antibody 87394-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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